top of page

Growth Conditions

Adapted from: Karas et al. (2015) Designer diatom episomes delivered by bacterial conjugation. https://doi.org/10.1038/ncomms7925

Screen Shot 2020-06-10 at 12.04.32 PM.pn

 

 

 

 

 

 

 

Strains

Screen Shot 2020-06-10 at 12.04.43 PM.pn
Screen Shot 2020-06-10 at 12.04.38 PM.pn

Current Strains

 

  • Commercially used UTEX strains

 

Available Here 

​

  • Other known wild type strains 

​

GenBank

​

Auxotrophic Strains

 

Auxotrophic Strains of P. tricornutum would reduce the use of traditional antibiotic selection, enable both positive and negative selection, and are essential for biocontainment of engineered P. tricornutum. Currently, known auxotrophic strains are:

 

  • -HIS

  • -URA

  • -TRP

​

A recent preprint by Slattery et al. presents a Cas9-generated method of generating a -URA strain of P. tricornutum.

​

 

 

 

 

 

 

 

 

 

 

 

 

 

Slattery et al. (2020) Cas9-generated auxotrophs of Phaeodactylum tricornutum are characterized by small and large deletions that can be complemented by plasmid-based genes.  https://doi.org/10.1101/2020.04.12.038471

​

​

Screen Shot 2020-05-21 at 12.59.52 AM.pn
bottom of page